Abstract
Four new indolosesquiterpenes, lecanindoles A-D (1-4), were isolated from fermentations of the terrestrial fungus Verticillium lecanii 6144. The structures of compounds 1-4 were elucidated from analysis of spectroscopic data. Compound 2 was reduced to give 4 and its isomer 5. Compound 4 was found to be a potent and selective progesterone receptor agonist with an EC50 of 1.1 +/- 0.4 nM in a cell-based luciferase reporter assay.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Chlorocebus aethiops
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Female
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Humans
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Hypocreales / chemistry*
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Indoles / chemistry
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Indoles / isolation & purification*
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Indoles / pharmacology
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Luciferases / metabolism
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Progestins / chemistry
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Progestins / isolation & purification*
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Progestins / pharmacology
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Receptors, Progesterone / agonists*
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Sesquiterpenes / chemistry
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Sesquiterpenes / isolation & purification*
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Sesquiterpenes / pharmacology
Substances
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Indoles
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Progestins
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Receptors, Progesterone
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Sesquiterpenes
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Luciferases